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A whole medium modify was done everyday, as well as the cells had been break up yet again as soon as a confluency of eighty% was reached. All undifferentiated iPSC and human ESC colonies had been preserved in Crucial 8 medium (E8 medium; Existence Systems) and incubated at 37°C in 5% CO2 humidified incubators. iPSCs and ESCs have been managed in T